Application
Western blot analysis of NET-2 expression in Hep G2 whole cell lysate.Application
Western blot analysis of NET-2 expression in SK-N-MC whole cell lysate.| Product Name | NET-2 Antibody (H-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of NET-2 of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | NET-2, also known as Tetraspanin-12 and Transmembrane 4 superfamily member 12, is a 305 amino acid multi-pass membrane protein that belongs to the transmembrane 4 superfamily, also known as the tetraspanin family. Members of the tetraspanin family are cell-surface proteins that are characterized by the presence of four hydrophobic domains and mediate signal transduction events that play a role in the regulation of cell development, activation, growth, motility, differentiation, and cancer. Considered molecular facilitators, tetraspanin proteins may regulate vesicle fusion and fission. Specifically, NET-2 plays a central role in retinal vascularization via regulation of NORRIN signal transduction. NET-2 also regulates membrane proteinases such as MT-MMP-1 and ADAM10. There are two isoforms of NET-2 which are produced as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of NET-2 of mouse rat and human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of NET-2 of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 23554 |
|---|
Application
Western blot analysis of NET-2 expression in Hep G2 whole cell lysate.
Application
Western blot analysis of NET-2 expression in SK-N-MC whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
