Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of FBXO7 expression in K-562 (A), C32 (B) and LADMAC (C) whole cell lysates.Application
Western blot analysis of FBXO7 expression in non-transfected: (A) and mouse FBXO7 transfected: (B) 293T whole cell lysates.| Product Name | FBXO7 Antibody (P-14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of FBXO7 of human origin |
| Modification Notes | F-box proteins are critical components of the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. They are members of a larger family of proteins that are involved in the regulation of a wide variety of cellular processes (including the cell cycle, immune responses, signaling cascades and developmental events) through the targeting of proteins, such as cyclins, cyclin-dependent kinase inhibitors, I˚B-å and ∫-catenin, for proteasomal degradation. FBXO7 (F-box protein 7), also known as FBX, FBX7 or PKPS, is a 522 amino acid protein that contains one F-box domain and functions as a component of the SCF complex. Defects in the gene encoding FBXO7 are associated with parkinsonian-pyramidal syndrome (PKPS), a hypokinetic rigid disorder that exhibits Parkinsonian and pyramidal-associated symptoms. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of FBXO7 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other FBX family proteins: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of FBXO7 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of FBXO7 of human origin |
| Entrez Gene | 25793 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of FBXO7 expression in K-562 (A), C32 (B) and LADMAC (C) whole cell lysates.
Application
Western blot analysis of FBXO7 expression in non-transfected: (A) and mouse FBXO7 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of FBXO7 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other FBX family proteins: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
