Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of decidual cells.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of trophoblastic cells.Application
Western blot analysis of eIF4AI/II expression in K-562 whole cell lysate.Application
Western blot analysis of eIF4AII expression in non-transfected 293T: (A), human eIF4AII transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of eIF4AI/II expression in mouse brain tissue extract.Application
Western blot analysis of eIF4AI expression in non-transfected 293T: (A), mouse eIF4AI transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of eIF4AI and eIF4AII expression in non-transfected: (A) and human eIF4AII transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).Application
Western blot analysis of eIF4AII expression in non-transfected 293: (A), human eIF4AII transfected 293: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of eIF4AI/II expression in HeLa whole cell lysate.| Product Name | eIF4AI/II Antibody (H-145) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 186-330 mapping within an internal region of eIF4AI of human origin |
| Modification Notes | Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a protein complex that contains eIF4A, eIF4E and eIF4G, binds mRNA at a 5’-cap motif and recruits the 43S ribosomal preinitiation complex to the eligible transcript. Along with eIF4B, the eIF4F complex mediates the unwinding of mRNA secondary structure to facilitate ribosome association. eIF4E specifically interacts with the 5’ cap, eIF4A(I,II) are bidirectional RNA helicases, and eIF4G(I,II) are scaffolding proteins which coordinate eIF4E, eIF4A, eIF3 and the 40S ribosome. Human eIF4AI (eIF4A, DDX2A) is a 406 amino acid protein that is 92.7% homologous to mouse eIF4AI. The promoter region of human eIF4A1 contains TATA and CAAT motifs and consensus binding sites to Sp1 and AP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of eIF4AI and eIF4AII of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 186-330 mapping within an internal region of eIF4AI of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 186-330 mapping within an internal region of eIF4AI of human origin |
| Entrez Gene | 1973 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of decidual cells.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of trophoblastic cells.
Application
Western blot analysis of eIF4AI/II expression in K-562 whole cell lysate.
Application
Western blot analysis of eIF4AII expression in non-transfected 293T: (A), human eIF4AII transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of eIF4AI/II expression in mouse brain tissue extract.
Application
Western blot analysis of eIF4AI expression in non-transfected 293T: (A), mouse eIF4AI transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of eIF4AI and eIF4AII expression in non-transfected: (A) and human eIF4AII transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).
Application
Western blot analysis of eIF4AII expression in non-transfected 293: (A), human eIF4AII transfected 293: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of eIF4AI/II expression in HeLa whole cell lysate.| Application Notes | recommended for detection of eIF4AI and eIF4AII of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
