Application
Western blot analysis of nov expression in non-transfected: (A) and human nov transfected: (B) 293T whole cell lysates.Application
Western blot analysis of nov expression in non-transfected: (A) and mouse nov transfected: (B) 293T whole cell lysates.Application
Western blot analysis of nov expression in non-transfected: (A) and human nov transfected: (B) 293 whole cell lysates.| Product Name | nov Antibody (H-71) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 48-118 (deletion at amino acid 104) mapping near the N-terminus of nov of human origin |
| Modification Notes | The CCN (CTGF/Cyr61/NOV) family of genes presently consists of six distinct members, which encode proteins that participate in fundamental biological processes such as cell proliferation, adhesion, migration, differentiation, wound healing, angiogenesis and several pathologies including fibrosis and tumorigenesis (1). Whereas Cyr61 and CTGF act as positive regulators of cell growth, nov (nephroblastoma overexpressed, CCN3 or NOVH) provides the first example of a CCN protein with negative regulatory properties and the first example of aberrant expression being associated with tumor development (1). In animals and humans increased expression of nov is detected in tissues where calcium is a key regulator, such as the adrenal gland, central nervous system, bone and cartilage, heart muscle and kidney (2,3). The nov protein associates with Notch1 extracellular domain and inhibits myoblast differentiation via Notch signaling pathway (4). The gene that expresses nov is located on human chromosome 8q24.12 and was originally cloned following discovery of its avian homolog as a consequence of overexpression in virally induced nephroblastoma (5) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of nov of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 48-118 (deletion at amino acid 104) mapping near the N-terminus of nov of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 48-118 (deletion at amino acid 104) mapping near the N-terminus of nov of human origin |
| Entrez Gene | 4856 |
|---|
Application
Western blot analysis of nov expression in non-transfected: (A) and human nov transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of nov expression in non-transfected: (A) and mouse nov transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of nov expression in non-transfected: (A) and human nov transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of nov of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
