Application
Western blot analysis of TH phosphorylation in untreated (A) and okadaic acid-treated (B) PC-12 whole cell lysates.| Product Name | p-TH Antibody (Ser 31) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 31 phosphorylated TH of mouse and rat origin by WB, IP and IF |
| Modification | Phosphoralated |
| Modification Notes | The enzyme tyrosine hydroxylase (TH), also designated tyrosine 3-monooxygenase (TY3H), catalyzes the conversion of tyrosine to L-DOPA. This is the rate limiting step in the biosynthesis of catecholamines such as dopamine, adrenalin and noradrenalin.Protein Kinase A (PKA) mediated phosphosphorylation at Serine 40 causes increased TH catalytic activity. Expressed mainly in the brain and adrenal glands, TH may play a role in the pathogenesis of Parkinson’s disease and the associated reduction in dopamine levels. The human tyrosine hydroxylase gene maps to 11p15. The phosphorylation of mouse and rat TH on Ser 31 by p35 leads to an increase in TH activity and stability. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIF |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 31 phosphorylated TH of mouse and rat origin by WB IP and IF |
|---|---|
| Tissue Specificity | recommended for detection of Ser 31 phosphorylated TH of mouse and rat origin by WB, IP and IF |
| Entrez Gene | 21823 |
|---|
Application
Western blot analysis of TH phosphorylation in untreated (A) and okadaic acid-treated (B) PC-12 whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
