Application
Western blot analysis of TH phosphorylation in purified non-phosphorylated rat recombinant TH fusion protein (A) and untreated PC-12 (B) and KCl-stimulated PC-12 (C) whole cell lysates.| Product Name | p-TH Antibody (Ser 19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 19 phosphorylated TH of rat origin by WB, IP and IF |
| Modification | Phosphoralated |
| Modification Notes | The enzyme tyrosine hydroxylase (TH), also designated tyrosine 3-monooxygenase (TY3H), catalyzes the conversion of tyrosine to L-DOPA. This is the rate limiting step in the biosynthesis of catecholamines such as dopamine, adrenalin and noradrenalin.Protein Kinase A (PKA) mediated phosphosphorylation at Serine 40 causes increased TH catalytic activity. Expressed mainly in the brain and adrenal glands, TH may play a role in the pathogenesis of Parkinson’s disease and the associated reduction in dopamine levels. The human tyrosine hydroxylase gene maps to 11p15. The phosphorylation of rat TH on Ser 19 by CaMKII leads to an increase in TH catalytic activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIF |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 19 phosphorylated TH of rat origin by WB IP and IF |
|---|---|
| Tissue Specificity | recommended for detection of Ser 19 phosphorylated TH of rat origin by WB, IP and IF |
Application
Western blot analysis of TH phosphorylation in purified non-phosphorylated rat recombinant TH fusion protein (A) and untreated PC-12 (B) and KCl-stimulated PC-12 (C) whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
