Application
Western blot analysis of NAT-2 expression in non-transfected: (A) and mouse NAT-2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of NAT-1/2 expression in HeLa (A), A-431 (B), A549 (C) and MOLT-4 (D) whole cell lysates.| Product Name | NAT-1/2 Antibody (H-52) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 117-168 mapping within an internal region of NAT-1 of human origin |
| Modification Notes | Arylamine N-acetyltransferases (NAT-1 and NAT-2) catalyze N- or O-acetylation of heterocyclic and arylamine substrates in the detoxification of a wide array of drugs. Certain alleles causing high levels of N-acetyltransferase activity have been associated with colon and urinary bladder cancers, as NATs also bioactivate several known carcinogens. Both NAT-1 and NAT-2 are cytoplasmic proteins and play an active role in the detoxification of many arylamine and hydrazine drugs. N-acetylation polymorphism is determined by the level of NAT activity in liver tissues, and has been linked to the action and toxicity of drugs that contain amines. Human NAT-1 is the functional homolog of rodent NAT-2, while human NAT-2 is the functional homolog of rodent NAT-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NAT-1 and NAT-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 117-168 mapping within an internal region of NAT-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 117-168 mapping within an internal region of NAT-1 of human origin |
| Entrez Gene | 9 |
|---|
Application
Western blot analysis of NAT-2 expression in non-transfected: (A) and mouse NAT-2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of NAT-1/2 expression in HeLa (A), A-431 (B), A549 (C) and MOLT-4 (D) whole cell lysates.| Application Notes | recommended for detection of NAT-1 and NAT-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
