Application
Western blot analysis of Fe65 expression in non-transfected: (A) and human Fe65 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Fe65 expression in non-transfected: (A) and human Fe65 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Fe65 expression in non-transfected: (A) and mouse Fe65 transfected: (B) 293T whole cell lysates.| Product Name | Fe65 Antibody (H-260) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-150 mapping at the N-terminus of Fe65 of human origin |
| Modification Notes | g-secretase cleaves the cell surface protein amyloid protein precursor (APP) at the transmembrane region into an extracelluar peptide (∫-Amyloid) and an intracelluar tail fragment. The cytoplasmic tail of APP forms a multimeric complex with Fe65 (also known as APBB1 for APP binding protein family B member 1). Specifically, Fe65 binds the YENPTY sequence in the cytoplasmic tail of APP. Fe65 is a nuclear adaptor protein widely expressed in the brain, including hippocampus and isocortex. In the cell, Fe65 and APP colocalize to the ER and golgi. The interaction between APP and Fe65 increases the translocation of APP to the cell surface and the subsequent secretion of ∫-Amyloid. Fe65 and APP localize with Mena, a cell-adhesion protein, and Fe65 regulates APP-dependent changes in cell motility. The gene encoding human Fe65 maps to chromosome 11p15. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Fe65 isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-150 mapping at the N-terminus of Fe65 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-150 mapping at the N-terminus of Fe65 of human origin |
| Entrez Gene | 322 |
|---|
Application
Western blot analysis of Fe65 expression in non-transfected: (A) and human Fe65 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Fe65 expression in non-transfected: (A) and human Fe65 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Fe65 expression in non-transfected: (A) and mouse Fe65 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Fe65 isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
