Application
Western blot analysis of CKIP-1 expression in non-transfected: (A) and human CKIP-1 transfected: (B) 293T whole cell lysates.| Product Name | CKIP-1 Antibody (H-240) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Casein Kinase II-interacting protein 1 (CKIP-1), also designated pleckstrin homology domain containing family O member 1 (PLEKHO1), is a 409-amino acid protein with an N-terminal pleckstrin homology domain and a putative C-terminal JUN leucine zipper interactive domain. CKIP-1 is expressed at the highest levels in skeletal muscle and heart, intermediately in placenta, lung and brain, and at the weakest levels in pancreas, liver and kidney. CKIP-1 localizes to the plasma membrane of transfected COS-7 cells and also to the plasma membrane and the nucleus in human osteosarcoma cells. It interacts with the N terminus of CSNK2A1 and with full length CSNK2A1, but not with CSNK2A2 or CSNK2B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CKIP-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 170-409 mapping at the C-terminus of CKIP-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 170-409 mapping at the C-terminus of CKIP-1 of human origin |
Application
Western blot analysis of CKIP-1 expression in non-transfected: (A) and human CKIP-1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of CKIP-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
