Application
Western blot analysis of RORα3 expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic localization.| Product Name | RORα Antibody (X-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of RORα of mouse, rat, human and zebrafish origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Modification Notes | Retinoids are metabolites of vitamin A (retinol) and represent an important class of signaling molecule during vertebrate development and tissue differentiation. A large group of nuclear transcription factors, including vitamin D3 receptor (VDR), thyroid hormone receptor (TR), RAR, RXR and ecdysone receptor, have a high affinity for retinoic acids and are members of the steroid receptor superfamily. This family acts by directly associating with DNA sequences known as hormone response elements (HREs) and bind DNA as either homo- or heterodimers. RORå is a member of the steroid receptor superfamily and is classified as an “orphan receptor” due to the lack of a defined ligand. Two isoforms of RORå have been described and are designated RORå1 and RORå2. RORå, also referred to as RZR, binds DNA as a monomer at consensus RORå response elements (ROREs). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of ROR&alpha of mouse rat human and zebrafish origin by WB IP IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of RORα of mouse, rat, human and zebrafish origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 6095 |
|---|
Application
Western blot analysis of RORα3 expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
