Application
Western blot analysis of Hemogen expression in RBL-1 whole cell lysate.| Product Name | Hemogen Antibody (R-190) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 191-380 mapping within an internal region of Hemogen of rat origin |
| Modification Notes | Hemogen (Hemopoietic gene protein, Erythroid differentiation-associated gene protein) is a 484 amino acid protein encoded by the human gene HEMGN. Hemogen is a nuclear protein that is expressed in hematopoietic precursor cells and can be detected in CD34+ and K-562 leukemia cell line. It is also expressed in bone marrow, testis, thymus and thyroid tumors, non-Hodgkin lymphoma, various leukemia cell lines, peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) of patients with leukemia. Hemogen is down-regulated during megakaryocytic differentiation of K-562 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA) (at protein level). It can be up-regulated in normal PBMCs by mitogens. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Hemogen of rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 191-380 mapping within an internal region of Hemogen of rat origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 191-380 mapping within an internal region of Hemogen of rat origin |
Application
Western blot analysis of Hemogen expression in RBL-1 whole cell lysate.| Application Notes | recommended for detection of Hemogen of rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
