Application
Western blot analysis of granzyme H expression in 293T whole cell lysate.Application
Western blot analysis of granzyme H expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic localization.| Product Name | granzyme H Antibody (T-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of granzyme H of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | The Granzyme family of proteins belong to the larger peptidase S1 family. Granzyme A and granzyme B are serine proteases that facilitate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Within the granules of activated CTLs, granzyme A and granzyme B are processed and converted to their active forms by the lysosomal cysteine protease cathepsin C. Once cleaved, these active proteases target distinct substrates for proteolysis and, thereby, mediate apoptosis through two different pathways. Granzyme H, also designated cytotoxic T-lymphocyte proteinase, cathepsin G-like 2 (CTSGL2) or cytotoxic serine protease C (CSP-C), contains one peptidase S1 domain. Granzyme H localizes to cytoplasmic granules of cytolytic T-lymphocytes and is important for target cell lysis in cell-mediated immune responses. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of granzyme H of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of granzyme H of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 2999 |
|---|
Application
Western blot analysis of granzyme H expression in 293T whole cell lysate.
Application
Western blot analysis of granzyme H expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
