Application
Western blot analysis of UDP-GlcDH expression in non-transfected: (A) and mouse UDP-GlcDH transfected: (B) 293T whole cell lysates.Application
Western blot analysis of UDP-GlcDH expression in non-transfected 293T: (A), human UDP-GlcDH transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Western blot analysis of UDP-GlcDH expression in Hep G2 whole cell lysate.| Product Name | UDP-Glucose-6 Dehydrogenase Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 195-494 mapping at the C-terminus of UDP-Glucose-6 of human origin |
| Modification Notes | UDP-GlcDH (also called UDP-glucose 6-dehydrogenase, UGDH or UDPGDH) is a member of the UDP-glucose/GDP-mannose dehydrogenase family. UDP-GlcDH converts UDP-glucose to UDP-glucuronic acid, which is a crucial component in the biosynthesis of the glycosaminoglycans, hyaluronan, heparan sulfate and chondroitin sulfate. Found as common components of the extracellular matrix, these glycosaminoglycans are significant in signal transduction, cell migration, cancer growth and cancer metastasis. UDP-glucuronic acid (UDP-GlcA) is needed in the liver for the excretion of toxic compounds. UDP-GlcDH is a ubiquitously expressed protein most abundant in the liver. The protein structure of UDP-GlcDH was first analyzed in cow liver and found to be a homohexamer. This structure is well conserved between species and phyla with an overall 97% sequence identity shared between different species of mammals. Research indicates that UDP-GlcDH expression is upregulated by TGF∫ and downregulated by hypoxia. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of UDP-Glucose-6 Dehydrogenase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 195-494 mapping at the C-terminus of UDP-Glucose-6 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 195-494 mapping at the C-terminus of UDP-Glucose-6 of human origin |
| Entrez Gene | 7358 |
|---|
Application
Western blot analysis of UDP-GlcDH expression in non-transfected: (A) and mouse UDP-GlcDH transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of UDP-GlcDH expression in non-transfected 293T: (A), human UDP-GlcDH transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Western blot analysis of UDP-GlcDH expression in Hep G2 whole cell lysate.| Application Notes | recommended for detection of UDP-Glucose-6 Dehydrogenase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
