Application
Western blot analysis of galectin-12 expression in non-transfected 293T: (A), mouse galectin-12 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.| Product Name | galectin-12 Antibody (H-166) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Galectin-12, also designated galectin-related inhibitor of proliferation 1 in mouse, is a 314 amino acid protein encoded by the human gene LGALS12. Galectin-12 is a member of the galectin family consisting of b-galactoside-binding proteins with conserved carbohydrate recognition domains. Galectin-12 binds lactose and may participate in the apoptosis of adipocytes. This protein is preferentially expressed in peripheral blood leukocytes and adipocytes. Galectin-12 is induced by cell cycle block at the G1 phase and causes G1 arrest when overexpressed. The galectin-12 gene is expressed in mouse preadipo-cytes and is upregulated when preadipocytes undergo cell cycle arrest, concomitant with acquisition of the competence to undergo differentiation in response to adipogenic hormone stimulation. Galectin-12 is an adipocyte-expressed protein which is downregulated by various insulin resistance-inducing hormones. As a result, galectin-12 may play a role in the pathogenesis of insulin resistance. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of galectin-12 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 171-336 mapping at the C-terminus of galectin-12 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 171-336 mapping at the C-terminus of galectin-12 of human origin |
| Entrez Gene | 85329 |
|---|
Application
Western blot analysis of galectin-12 expression in non-transfected 293T: (A), mouse galectin-12 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.| Application Notes | recommended for detection of galectin-12 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
