Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.Application
Western blot analysis of CHMP4B expression in NIH/3T3 whole cell lysate.| Product Name | CHMP4B Antibody (C-12) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The charged multivesicular body proteins or chromatin modifying proteins, commonly designated CHMPs, belong to the vacuolar sorting protein family and function as chromatin-modifying proteins. CHMP1-6 are all components of ESCRT (endosomal sorting complex required for transport) I, II or III complexes. These complexes are crucial for sorting endosomal articles into multivesicular bodies (MVBs), and are also required for the formation of these bodies. CHMP4B (charged multivesicular body protein 4b) is a 224 amino acid protein that interacts with Alix, which results in the redistribution of Alix from the cytoplasm to the perinuclear area. Overexpression of CHMP4B induces accumulation of ubiquinated proteins and inhibits degradation of endocytosed EGF. Defects in the gene encoding CHMP4B can cause posterior polar cataract type 3 (CTPP3), a disease characterized by distinct opacity located at the back of the lens resulting in visual impairment and eventual blindness. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CHMP4B of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other CHMP family members also reactive with additional species, including porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of CHMP4B of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of CHMP4B of human origin |
| Entrez Gene | 128866 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.
Application
Western blot analysis of CHMP4B expression in NIH/3T3 whole cell lysate.| Application Notes | recommended for detection of CHMP4B of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other CHMP family members also reactive with additional species, including porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
