Application
Antibodies tested include p-Bad (Ser 112): (A, B, C) and Bad (H-168): (D, E, F).Application
Western blot analysis of Bad phosphorylation in non-transfected: (A), untreated human Bad transfected: (B) and lambda protein phosphatase treated human Bad transfected: (C) 293T whole cell lysates.Application
Western blot analysis of Bad phosphorylation in non-transfected: (A) and human Bad transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue extract showing cytoplasmic staining.Application
Western blot analysis of phosphorylated Bad expression in untreated (A) and forskolin-treated (B) 293 whole cell lysates.| Product Name | p-Bad Antibody (Ser 112) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Phosphorylation of BAD, a pro-apoptotic member of the Bcl-2 protein family, on either serine 112 or serine 136 is thought to be necessary and sufficient for growth factors to promote cell survival (1–3). Serine 155 is a major site of phosphorylation by protein kinase A (PKA) and serum-induced kinases (1). Serine 155 phosphorylation requires the prior phosphorylation of serine 136, which recruits 14-3-3 proteins that then function to increase the accessibility of serine 155 to survival-promoting kinases (4). Like serine 112 and serine 136, phosphorylation of serine 155 inhibits the pro-apoptotic function of BAD (1). Serine 155 phosphorylation disrupts the binding of BAD to prosurvival Bcl-2 proteins and thereby promotes cell survival (4,5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 112 phosphorylated Bad of mouse origin, correspondingly phosphorylated Ser 75 of human origin and correspondingly phosphorylated Ser 113 of rat origin by WB, IP, IF and IHC(P) | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 112 of Bad of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 112 of Bad of human origin |
| Entrez Gene | 572 |
|---|
Application
Antibodies tested include p-Bad (Ser 112): (A, B, C) and Bad (H-168): (D, E, F).
Application
Western blot analysis of Bad phosphorylation in non-transfected: (A), untreated human Bad transfected: (B) and lambda protein phosphatase treated human Bad transfected: (C) 293T whole cell lysates.
Application
Western blot analysis of Bad phosphorylation in non-transfected: (A) and human Bad transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue extract showing cytoplasmic staining.
Application
Western blot analysis of phosphorylated Bad expression in untreated (A) and forskolin-treated (B) 293 whole cell lysates.| Application Notes | recommended for detection of Ser 112 phosphorylated Bad of mouse origin, correspondingly phosphorylated Ser 75 of human origin and correspondingly phosphorylated Ser 113 of rat origin by WB, IP, IF and IHC(P) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
