Application
Western blot analysis of GM2/GD2 Synthase expression in rat brain (A) and mouse brain (B) tissue extracts.| Product Name | GM2/GD2 Synthase Antibody (H-180) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | GM2/GD2 synthase is a 533 amino acid protein encoded by the human gene B4GALNT1. The GM2 and GD2 gangliosides are sialic acid-containing glyco-sphingolipids that play a role in signal transduction and cell-cell recognition. GM2/GD2 synthase is expressed abundantly in normal brain tissue of vertebrates. It contains a single 18 amino acid hydrophobic segment near the amino-terminus flanked by basic residues. GM2/GD2 synthase primarily controls the balance between expression of simple and complex gangliosides at the cell surface. The ganglioside GD2 is expressed using GM2/GD2 synthase in almost all neuroblastomas (NBs) as well as other neuroectoderm-derived tumor cells, such as malignant melanoma, adult T cell leukemia and some colon and gastric cancers. GM2/GD2 synthase is a useful marker for NBs, and may aid in evaluating adjuvant treatment efficacy in neuroblastoma with prognostic potential. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GM2/GD2 Synthase of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including canine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-180 mapping at the N-terminus of GM2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-180 mapping at the N-terminus of GM2 of human origin |
| Entrez Gene | 2583 |
|---|
Application
Western blot analysis of GM2/GD2 Synthase expression in rat brain (A) and mouse brain (B) tissue extracts.| Application Notes | recommended for detection of GM2/GD2 Synthase of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including canine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
