Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.| Product Name | p-A-Raf Antibody (Ser 299) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 299 of A-Raf of human origin |
| Modification | Phosphoralated |
| Modification Notes | Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/ threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue. Active human A-Raf is designated p-A-Raf and is phosphorylated on Serine residue 299. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFELISA |
| recommended for detection of Ser 299 phosphorylated A-Raf of human origin and correspondingly phosphorylated Ser 297 A-Raf of mouse and rat origin of mouse, rat and human origin by WB, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 299 of A-Raf of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 299 of A-Raf of human origin |
| Entrez Gene | 369 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.| Application Notes | recommended for detection of Ser 299 phosphorylated A-Raf of human origin and correspondingly phosphorylated Ser 297 A-Raf of mouse and rat origin of mouse, rat and human origin by WB, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
