Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human smooth muscle tissue showing cytoplasmic staining of smooth muscle cells.Application
Western blot analysis of PI 3-kinase C2α expression in HeLa (A), Jurkat (B) and IMR-32 (C) whole cell lysates.| Product Name | PI 3-kinase C2α Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 61-360 mapping near the N-terminus of PI 3-kinase C2α of human origin |
| Modification Notes | Phosphatidylinositol 3-kinases (PI3Ks) phosphorylate the 3' OH position of the inositol ring of inositol lipids. Human PI 3-kinase C2a (PIK3C2A, C2-containing phosphatidylinositol kinase, p110a or CPK) contains a C-terminal calcium-binding and phospholipid-binding module known as the C2 domain. The cDNA sequence of PI 3-kinase C2a predicts a 1,686-amino acid protein that is 90% identical to mouse Cpk (term for the Drosophila homolog). Northern blot analysis reveals that PI 3-kinase C2a is expressed as an 8 kb mRNA in a wide variety of tissues. In vitro, the PI 3-kinase C2a enzyme can phosphorylate phospha-tidylinositol and phosphatidylinositol-4-phosphate. The PI 3-kinase C2a gene contains 32 exons and spans approximately 76 kb. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PI 3-kinase C2α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 61-360 mapping near the N-terminus of PI 3-kinase C2&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 61-360 mapping near the N-terminus of PI 3-kinase C2α of human origin |
| Entrez Gene | 5286 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human smooth muscle tissue showing cytoplasmic staining of smooth muscle cells.
Application
Western blot analysis of PI 3-kinase C2α expression in HeLa (A), Jurkat (B) and IMR-32 (C) whole cell lysates.| Application Notes | recommended for detection of PI 3-kinase C2α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
