Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal and glial cells.Application
Western blot analysis of GADD 45γ expression in non-transfected: (A) and mouse GADD 45γ transfected: (B) 293T whole cell lysates.| Product Name | GADD 45γ Antibody (H-65) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 95-159 mapping at the C-terminus of GADD 45γ of human origin |
| Modification Notes | Cell cycle progression is subject to arrest at G1 and G2 checkpoints in response to DNA damage, presumably to allow time for DNA repair prior to entry into S and M phase, respectively. The p53 tumor suppressor is required for one such G1 checkpoint and functions to upregulate expression of GADD 45 and p21. GADD 45 binds both Cdks and PCNA, a protein involved in DNA replication and repair. GADD 45 stimulates DNA excision repair in vitro and in hibits entry of cells into S phase. Thus, it has been suggested that GADD 45 may serve as a link between the p53-dependent cell cycle checkpoint and DNA repair. GADD 45-like proteins, GADD 45∫ and GADD 45©, have been shown to be induced by environmental stresses. GADD 45∫ and GADD 45© are thought to induce p38/JNK activation via MEKK4 activation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GADD 45γ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 95-159 mapping at the C-terminus of GADD 45&gamma of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 95-159 mapping at the C-terminus of GADD 45γ of human origin |
| Entrez Gene | 10912 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal and glial cells.
Application
Western blot analysis of GADD 45γ expression in non-transfected: (A) and mouse GADD 45γ transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of GADD 45γ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
