Application
Immunoper-oxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing nuclear staining of squamous epithelial cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of p68 RNA Helicase expression in non-transfected 293T: (A), human p68 RNA Helicase transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of p68 RNA Helicase expression in non-transfected 293T: (A), mouse p68 RNA Helicase transfected 293T: (B) and Jurkat (C) whole cell lysates.| Product Name | p68 RNA Helicase Antibody (H-144) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | p68 RNA helicase is a nuclear protein that exhibits RNA-dependent ATPase activity. Phosphorylation by protein kinase C inhibits p68 RNA helicase activity. p68 RNA helicase appears to play a role in organ differentiation during development. Furthermore, p68 RNA helicase is expressed in early neural development and in various mesodermal tissues in a number of different chordate embryos. At the cellular level, the expression levels of p68 RNA helicase increases in serum-induced quiescent cell lines. p68 RNA helicase may function as a coactivator for estrogen receptor alpha. Additionally, p68 RNA helicase associates with transcriptional coactivators CBP and p300. p68 RNA helicase localizes to the nucleus under normal conditions. During late telophase, p68 RNA helicase and fibrillarin colocalize to nascent nucleoli. p68 RNA helicase may function as a heterodimer with p72 RNA helicase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of p68 RNA Helicase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 471-614 mapping at the C-terminus of p68 RNA Helicase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 471-614 mapping at the C-terminus of p68 RNA Helicase of human origin |
| Entrez Gene | 1655 |
|---|
Application
Immunoper-oxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing nuclear staining of squamous epithelial cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of p68 RNA Helicase expression in non-transfected 293T: (A), human p68 RNA Helicase transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of p68 RNA Helicase expression in non-transfected 293T: (A), mouse p68 RNA Helicase transfected 293T: (B) and Jurkat (C) whole cell lysates.| Application Notes | recommended for detection of p68 RNA Helicase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
