Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear and cytoplasmic staining of decidual cells.Application
Western blot analysis of FKHR expression in non-transfected 293: (A), mouse FKHR transfected 293: (B) and CTLL-2 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear bodies localization.Application
Western blot analysis of FKHR expression in non-transfected 293: (A), mouse FKHR transfected 293: (B) and TE 671 (C) whole cell lysates.| Product Name | FKHR Antibody (H-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 471-600 mapping near the C-terminus of FKHR of human origin |
| Modification Notes | FKHR (for forkhead in rhabdomyosarcoma) and FKHRL1 are members of the forkhead family of transcription factors. Transcriptional activation of FKHR proteins is regulated by the serine/threonine kinase Akt1, which phosphorylates FKHRL1 and results in FKHRL1 associating with 14-3-3 proteins and being retained in the cytoplasm. Induction of apoptosis or withdrawal of growth factors stimulates dephosphorylation and nuclear translocation of FKHR proteins, leading to FKHR-induced gene-specific transcriptional activation. FKHR, also designated forkhead box protein O1A (FOXO1), is a ubiquitously expressed protein that shuttles between the cytoplasm and nucleus. Genetic mutations in FKHR genes, including the t(2;13) and t(1;3) translocations, are commonly found in alveolar rhabdomyosarcomas. These translocations result in the fusion of the amino terminus of Pax-3 or Pax-7, including the paired box and homeodomain DNA-binding domains, with the carboxy-terminus of FKHR, which contains a transcriptional activation domain. The Pax-3/FKHR fusion protein appears to function as an oncogenic transcription factor that enhances the activation of normal Pax-3 target genes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of FKHR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 471-600 mapping near the C-terminus of FKHR of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 471-600 mapping near the C-terminus of FKHR of human origin |
| Entrez Gene | 2308 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear and cytoplasmic staining of decidual cells.
Application
Western blot analysis of FKHR expression in non-transfected 293: (A), mouse FKHR transfected 293: (B) and CTLL-2 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear bodies localization.
Application
Western blot analysis of FKHR expression in non-transfected 293: (A), mouse FKHR transfected 293: (B) and TE 671 (C) whole cell lysates.| Application Notes | recommended for detection of FKHR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
