Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of Legumain expression in non-transfected: (A) and human Legumain transfected: (B) 293 whole cell lysates.Application
Western blot analysis of Legumain expression in non-transfected 293T: (A), human Legumain transfected 293T: (B) and JEG-3 (C) whole cell lysates.Application
Western blot analysis of Legumain expression in KNRK whole cell lysate.Application
Western blot analysis of Legumain expression in JEG-3 whole cell lysate.Application
Western blot analysis of Legumain expression in KNRK (A) and Caki-1 (B) whole cell lysates.| Product Name | Legumain Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 21-320 mapping near the N-terminus of Legumain of human origin |
| Modification Notes | Legumain, also known as LGMN, AEP (asparaginyl endopeptidase) or PRSC1, is a 433 amino acid protein that localizes to the lysosome and belongs to the peptidase C13 family. Expressed ubiquitously with particularly high expression in placenta, heart and kidney, Legumain functions as a cysteine protease that specifically catalyzes the hydrolysis of asparaginyl and aspartyl bonds. Additionally, Legumain is thought to be involved in the processing of bacterial proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Legumain exists as both a precursor and a fully mature, active enzyme that is produced in dendritic cells. Overexpression of Legumain may be associated with the formation of solid tumors, suggesting a role for Legumain in carcinogenesis. Multiple isoforms of Legumain exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of precursor and mature Legumain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 21-320 mapping near the N-terminus of Legumain of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 21-320 mapping near the N-terminus of Legumain of human origin |
| Entrez Gene | 5641 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of Legumain expression in non-transfected: (A) and human Legumain transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of Legumain expression in non-transfected 293T: (A), human Legumain transfected 293T: (B) and JEG-3 (C) whole cell lysates.
Application
Western blot analysis of Legumain expression in KNRK whole cell lysate.
Application
Western blot analysis of Legumain expression in JEG-3 whole cell lysate.
Application
Western blot analysis of Legumain expression in KNRK (A) and Caki-1 (B) whole cell lysates.| Application Notes | recommended for detection of precursor and mature Legumain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
