Application
Western blot analysis of HoxD10 expression in non-transfected 293T: (A), mouse HoxD10 transfected 293T: (B) and ECV304 (C) whole cell lysates.Application
Western blot analysis of HoxD10 expression in non-transfected: (A) and mouse HoxD10 transfected: (B) 293T whole cell lysates.| Product Name | HoxD10 Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 21-100 mapping near the N-terminus of HoxD10 of human origin |
| Modification Notes | The Hox proteins play a role in development and cellular differentiation by regulating downstream target genes. Specifically, the Hox proteins direct DNA-protein and protein-protein interactions that assist in determining the morphologic features associated with the anterior-posterior body axis. Hox proteins are involved in controlling axial patterning, leukaemias and hereditary malformations. Homeobox protein HoxD10, also designated Hox-4D or Hox-4E, belongs to the Abd-B homeobox familyi of proteins. HoxD10 is a nuclear protein primarily expressed in the adult male and female urogenitcal tracts but also expressed in developing limb buds during development. Defects in the gene encoding for the HoxD10 protein cause congentital vertical talus (CVT), more commonly known as rocker-bottom foot deformity. CVT is characterized by a dislocation of the talonavicular joint but is usually accompanied by other congenital deformities. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of HoxD10 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 21-100 mapping near the N-terminus of HoxD10 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 21-100 mapping near the N-terminus of HoxD10 of human origin |
| Entrez Gene | 3236 |
|---|
Application
Western blot analysis of HoxD10 expression in non-transfected 293T: (A), mouse HoxD10 transfected 293T: (B) and ECV304 (C) whole cell lysates.
Application
Western blot analysis of HoxD10 expression in non-transfected: (A) and mouse HoxD10 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of HoxD10 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
