Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gail bladder tissue showing cytoplasmic staining of glandular cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Calpain 3 expression in mouse brain tissue extract.| Product Name | Calpain 3 Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Calpain, an intracellular calcium-dependent protease that cleaves cytoskeletal and submembranous proteins, plays a role in cytoskeletal reorganization and muscle protein degradation (1). Calpain is a heterodimer composed of a small regulatory subunit and one of three large catalytic subunits, designated calpain 1, calpain 2 and calpain 3 (2). Calpain 3 (calpain p94) is a muscle-preferred calcium activated neutral protease that localizes to the nucleus (3). The gene encoding human calpain 3 maps to chromosome 15q15.1-q21.1 (4). Mutations involving the calpain 3 gene are associated with limb-girdle muscle dystrophy type 2A, a form of autosomal recessive and progressive neuromuscular disorder (4). Calpastatin regulates calpain by inhibiting both the proteolytic activity of calpain and its binding to membranes (5,6). Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing (7,8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Calpain 3 isoforms I, II and III of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-80 mapping at the N-terminus of Calpain 3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-80 mapping at the N-terminus of Calpain 3 of human origin |
| Entrez Gene | 825 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gail bladder tissue showing cytoplasmic staining of glandular cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Calpain 3 expression in mouse brain tissue extract.| Application Notes | recommended for detection of Calpain 3 isoforms I, II and III of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
