Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Product Name | Dimethyl Histone H3 Antibody (Lys 80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | Dimethyl Lys 80 |
| Modification Notes | Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIF |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of lysine 80 dimethylated Histone H3 of mammalian and yeast origin by WB IP and IF non cross-reactive with monomethylated trimethylated and nonmethylated Histone H3 |
|---|---|
| Tissue Specificity | recommended for detection of lysine 80 dimethylated Histone H3 of mammalian and yeast origin by WB, IP and IF; non cross-reactive with monomethylated, trimethylated, and nonmethylated Histone H3 |
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
