Application
Immunofluorescence staining of methanol-fixed HeLa cells membrane localization.| Product Name | Podocalyxin-like 1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 23-322 mapping within an N-terminal extracellular domain of Podocalyxin-like 1 of human origin |
| Modification Notes | Sialomucins are a family of cell adhesion molecules that mediate the interaction between leukocytes and endothelial cells during the inflammatory process. Podocalyxin-like protein 1 (PCLP1), a member of the sialomucin family, is a transmembrane glycoprotein and is structurally related to the L-Selectin ligand, CD34. Podocalyxin-like protein 1 encodes a 21 amino acid N-terminal signal peptide and a 26 amino acid transmembrane region. The extracellular domain contains sites for N- and O-linked glycosylation and the intracellular domain has several potential phosphorylated sites. Podocalyxin-like protein 1 is expressed on podocyte foot processes, where it maintains the glomerular filtration barrier. It is also expressed in endo-thelial cells as well as hemangioblasts, a precursor of hematopoietic stem cells (HSC). Subsequently, Podocalyxin-like protein 1 is thought to be an appropriate marker for hemagioblast detection. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Podocalyxin-like 1 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 23-322 mapping within an N-terminal extracellular domain of Podocalyxin-like 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 23-322 mapping within an N-terminal extracellular domain of Podocalyxin-like 1 of human origin |
| Entrez Gene | 5420 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells membrane localization.| Application Notes | recommended for detection of Podocalyxin-like 1 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
