Application
Western blot analysis of SPTLC1 expression in non-transfected: (A) and mouse SPTLC1 transfected: (B) 293T whole cell lysates and mouse liver tissue extract (C).Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of trophoblastic cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.| Product Name | SPTLC1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 174-473 mapping at the C-terminus of SPTLC1 of human origin |
| Modification Notes | SPTLC1 (serine palmitoyltransferase 1), also known as LCB1, and SPTLC2 (serine palmitoyltransferase 2), also known as LCB2, together catalyze sphingolipid biosynthesis by converting L-serine and palmitoyl-CoA to 3-oxosphinganine, utilizing pyridoxal 5'-phosphate as a cofactor. Increases in transepidermal water loss triggers upregulation of serine palmitoyltransferase mRNA expression in humans. Deficiencies in wild type SPTLC1 and SPTLC2 can lead to hereditary sensory neuropathy, atopic eczema, and psoriasis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of SPT1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 174-473 mapping at the C-terminus of SPTLC1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 174-473 mapping at the C-terminus of SPTLC1 of human origin |
| Entrez Gene | 10558 |
|---|
Application
Western blot analysis of SPTLC1 expression in non-transfected: (A) and mouse SPTLC1 transfected: (B) 293T whole cell lysates and mouse liver tissue extract (C).
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of trophoblastic cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.| Application Notes | recommended for detection of SPT1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
