Application
Western blot analysis of cadherin-23 expression in non-transfected: (A) and truncated human cadherin-23 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of cadherin-23 expression in non-transfected: (A) and truncated human cadherin-23 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of normal mouse skin frozen section showing membrane staining.| Product Name | cadherin-23 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 151-450 mapping within an extracellular domain of cadherin-23 of human origin |
| Modification Notes | Cadherin-23 represents the first in this family of calcium binding proteins of which mutations in the extracellular calcium binding domain contribute to an inherited disorder, Usher syndrome type 1D (USH1D). Patients with USH1D exhibit congenital sensorineural hearing loss, vestibular dysfunction, and visual impairment due to early onset of retinitis pigmentosa (RP). In the inner ear, cadherin-23 interacts with myosin VIIIa and harmonin to form a functional network duing hair cell differentiation, and in the retina to assemble a supra-molecular complex contributing to the organization of the cytoskeletal matrices of the pre- and post-synaptic region. A number of cadherin-23 splice variants exist in association with various phenotypic expression, indicating that differential mutations result in variable presentation of the disease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of cadherin-23 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 151-450 mapping within an extracellular domain of cadherin-23 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 151-450 mapping within an extracellular domain of cadherin-23 of human origin |
| Entrez Gene | 64072 |
|---|
Application
Western blot analysis of cadherin-23 expression in non-transfected: (A) and truncated human cadherin-23 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of cadherin-23 expression in non-transfected: (A) and truncated human cadherin-23 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of normal mouse skin frozen section showing membrane staining.| Application Notes | recommended for detection of cadherin-23 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
