Application
Western blot analysis of Calpain 5 expression in non-transfected: (A) and human Calpain 5 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Calpain 5 expression in non-transfected: (A) and mouse Calpain 5 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Calpain 5 expression in TF-1 whole cell lysate.Application
Western blot analysis of human recombinant Calpain 5 fusion protein.| Product Name | Calpain 5 Antibody (H-105) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-105 mapping at the N-terminus of Calpain 5 of human origin |
| Modification Notes | Calpains are calcium-activated thiol proteases involved in intracellular processing of proteins and signal transduction. The classic calpains are hetero-dimers with one large subunit, one small subunit and five EF-hand-calcium binding structures. The large subunit varies between family members and can be active without the small subunit. Calpain 5, sometimes referred to as HTRA3, belongs to the non-classical calpain subfamily that lacks the EF-hand-calcium-binding structures. Calpain 5 is ubiquitously expressed, localizes to the cytoplasm and nucleus and is a homolog of Calpain 10. Calpain 5, in addition to Calpain 10 and Calpain 6, differs from other subfamily members by the presence of a T-domain that is homologous to the Caenorhabditis elegans protein Tra-3. Calpain 5, the human ortholog of this nematode protein, may play a role in sex determination. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Calpain 5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-105 mapping at the N-terminus of Calpain 5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-105 mapping at the N-terminus of Calpain 5 of human origin |
| Entrez Gene | 726 |
|---|
Application
Western blot analysis of Calpain 5 expression in non-transfected: (A) and human Calpain 5 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Calpain 5 expression in non-transfected: (A) and mouse Calpain 5 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Calpain 5 expression in TF-1 whole cell lysate.
Application
Western blot analysis of human recombinant Calpain 5 fusion protein.| Application Notes | recommended for detection of Calpain 5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
