Application
Western blot analysis of PLCδ1 expression in non-transfected: (A) and mouse PLCδ1 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing membrane and cytoplasmic staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of PLC δ1 expression in A-10 (A), F9 (B) and NTERA-2 cl.D1 (C) whole cell lysates.| Product Name | PLC δ1 Antibody (H-140) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-140 mapping at the N-terminus of PLC δ1 of human origin |
| Modification Notes | Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor-mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) from phosphatidylinositol 4,5-bisphosphate. There are several mammalian PLC proteins, including PLC ∫1, PLC ∫2, PLC ∫3, PLC ∫4, PLC©1, PLC©2, PLC ∂1, PLC ∂3, PLC ∂4 and PLCe. PLC ∂1, a calcium signal amplifier, is activated by an atypical GTP-binding protein and functions as an effector for GTP-binding protein transglutaminase II-mediated oxytocin receptor and å1B-adrenoreceptor signaling. PLC ∂1 is highly expressed in brain, heart, lung and testis and is abnormally accumulated in autopsied brains with Alzheimer’s disease (AD), suggesting that it may play a role in the pathology of AD. Both PLC ∂3 and PLC ∂4 contain several functional domains through which they bind calcium as a cofactor and catalyze the creation of DAG and IP3, playing an essential role in signal transduction. PLC ∂4 is highly expressed in skeletal muscle and kidney tissue, as well as in corneal epithelial cells, suggesting a role in the regulation of kidney and ocular function. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PLC δ1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-140 mapping at the N-terminus of PLC &delta 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-140 mapping at the N-terminus of PLC δ1 of human origin |
| Entrez Gene | 5333 |
|---|
Application
Western blot analysis of PLCδ1 expression in non-transfected: (A) and mouse PLCδ1 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing membrane and cytoplasmic staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of PLC δ1 expression in A-10 (A), F9 (B) and NTERA-2 cl.D1 (C) whole cell lysates.| Application Notes | recommended for detection of PLC δ1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
