Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Product Name | NEDD8 Antibody (FL-81) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-81 representing full length NEDD8 of human origin |
| Modification Notes | NEDD8 is a ubiquitin (Ub)-like molecule that shares 80% homology with ubiquitin, a protein known to modify and target various proteins for proteolytic degradation. NEDD8 and the corresponding yeast homolog Rub1 are activated by the E1 ubiquitin activating enzyme UBA2, which forms isopeptide linkages between thio esters. Similar to the Ub-mediated proteolytic pathway, NEDD8 is covalently coupled to an E3 Ub ligase by the E2 conjugating enzyme, Ubc12. NEDD8 preferentially associates with the CUL-2 protein in the E3 ligase complex CBCVHL, which consists of cullin proteins associating with elongin B/C and the tumor suppressor F-box protein, von Hippel Lindau (VHL). NEDD8 is predominantly localized to the nucleus and is highly expressed in adult heart and skeletal muscle. In vitro studies indicate that NEDD8 tetramers are also able to bind to the 26S Proteasome, and that they assemble into complexes with conserved Ub-like moieties, suggesting that NEDD8 may regulate proteolysis of intracellular proteins similar to other Ub-mediated pathways. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NEDD8 of mouse, rat and human origin by WB, IP, IF and ELISA partially cross-reactive with ubiquitin and other ubiquitin-like proteins also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-81 representing full length NEDD8 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-81 representing full length NEDD8 of human origin |
| Entrez Gene | 4738 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Application Notes | recommended for detection of NEDD8 of mouse, rat and human origin by WB, IP, IF and ELISA partially cross-reactive with ubiquitin and other ubiquitin-like proteins also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
