Application
Western blot analysis of PLC δ4 expression in non-transfected: (A) and human PLC δ4 transfected: (B) 293T whole cell lysates and rat testis tissue extract (C).Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of PLC δ4 expression in rat testis (A) and rat skeletal muscle (B) tissue extracts.| Product Name | PLC δ4 Antibody (H-250) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-250 mapping at the N-terminus of PLC δ4 of human origin |
| Modification Notes | Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor-mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) from phosphatidylinositol 4,5-bisphosphate. There are several mammalian PLC proteins, including PLC ∫1, PLC ∫2, PLC ∫3, PLC ∫4, PLC©1, PLC©2, PLC ∂1, PLC ∂3, PLC ∂4 and PLCe. PLC ∂1, a calcium signal amplifier, is activated by an atypical GTP-binding protein and functions as an effector for GTP-binding protein transglutaminase II-mediated oxytocin receptor and å1B-adrenoreceptor signaling. PLC ∂1 is highly expressed in brain, heart, lung and testis and is abnormally accumulated in autopsied brains with Alzheimer’s disease (AD), suggesting that it may play a role in the pathology of AD. Both PLC ∂3 and PLC ∂4 contain several functional domains through which they bind calcium as a cofactor and catalyze the creation of DAG and IP3, playing an essential role in signal transduction. PLC ∂4 is highly expressed in skeletal muscle and kidney tissue, as well as in corneal epithelial cells, suggesting a role in the regulation of kidney and ocular function. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PLC δ4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-250 mapping at the N-terminus of PLC &delta 4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-250 mapping at the N-terminus of PLC δ4 of human origin |
| Entrez Gene | 84812 |
|---|
Application
Western blot analysis of PLC δ4 expression in non-transfected: (A) and human PLC δ4 transfected: (B) 293T whole cell lysates and rat testis tissue extract (C).
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of PLC δ4 expression in rat testis (A) and rat skeletal muscle (B) tissue extracts.| Application Notes | recommended for detection of PLC δ4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
