Application
Western blot analysis of creatine kinase-M/B expression in A-673 whole cell lysate.Application
Western blot analysis of creatine kinase-M expression in non-transfected: (A) and human creatine kinase-M transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic staining.Application
Western blot analysis of creatine kinase-M expression in mouse heart tissue extract.Application
Western blot analysis of creatine kinase-M and creatine kinase-B expression in rat skeletal muscle tissue extract.| Product Name | creatine kinase-M/B Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 136-185 mapping within an internal region of creatine kinase-M of human origin |
| Modification Notes | Creatine kinases (CKs) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. Creatine kinases provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems including muscle, electrocytes, retina photoreceptor cells, brain cells, kidney, salt glands, myometrium, placenta, pancreas, thymus, thyroid, intestinal epithelial cells, endothelial cells, cartilage and bone cells, macrophages, blood platelets, and tumor and cancer cells. Human cytoplasmic creatine kinase-B, also designated CK-B and BCK, is a 381 amino acid, brain tissue- specific isoform of creatine kinase. Human cytoplasmic creatine kinase-M (CK-M, MCK) is a muscle tissue-specific isoform of creatine kinase. Human cytoplasmic creatine kinase-Mi (Mi-CK, MtCK) is a 416 amino acid mitochondrial-specific isoform of creatine kinase. Cytosolic creatine kinases are important in the energetic regulation of Ca2+-pumps and in the maintenance of Ca2+-homeostasis . |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of creatine kinase-M and creatine kinase-B chains of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 136-185 mapping within an internal region of creatine kinase-M of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 136-185 mapping within an internal region of creatine kinase-M of human origin |
| Entrez Gene | 1158 |
|---|
Application
Western blot analysis of creatine kinase-M/B expression in A-673 whole cell lysate.
Application
Western blot analysis of creatine kinase-M expression in non-transfected: (A) and human creatine kinase-M transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic staining.
Application
Western blot analysis of creatine kinase-M expression in mouse heart tissue extract.
Application
Western blot analysis of creatine kinase-M and creatine kinase-B expression in rat skeletal muscle tissue extract.| Application Notes | recommended for detection of creatine kinase-M and creatine kinase-B chains of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
