Application
Western blot analysis of N-Shc expression in SK-N-SH nuclear extract (A) and SH-SY5Y (B) and H4 (C) whole cell lysates.| Product Name | N-Shc Antibody (H-140) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 191-330 mapping within an internal region of N-Shc of human origin |
| Modification Notes | Src homology (SH2) domains are noncatalytic sequences that are conserved among a number of cytoplasmic signaling proteins. These signaling proteins are directly regulated by receptor tyrosine kinases and control the activation of mitogenic signal transduction pathways by such receptors. For instance, ligand-induced activation of the EGF and PDGF receptors induces dimerization, triggers receptor autophosphorylation on tyrosine residues and results in the binding of a number of cytoplasmic SH2 domain proteins such as PLC g1, Ras GAP and PI 3-kinase to the activated receptors. Another gene, Shc, encodes two proteins with a single SH2 domain. A Shc-related gene N-Shc (for neuronal Shc), encodes a protein that contains two phosphotyrosine domains (PTB), a single SH2 domain and is expressed exclusively in the brain. Neither Shc nor N-Shc have any identifiable catalytic activity, suggesting them to be members of an expanding class of proteins that function to couple activated growth factor receptors to downstream signaling intermediates. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of N-Shc p52 and p64 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 191-330 mapping within an internal region of N-Shc of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 191-330 mapping within an internal region of N-Shc of human origin |
| Entrez Gene | 53358 |
|---|
Application
Western blot analysis of N-Shc expression in SK-N-SH nuclear extract (A) and SH-SY5Y (B) and H4 (C) whole cell lysates.| Application Notes | recommended for detection of N-Shc p52 and p64 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
