Application
Western blot analysis of KIF3B expression in A2058 (A), T-47D (B) and Caki-1 (C) whole cell lysates and rat brain (D) and mouse brain (E) tissue extracts.| Product Name | KIF3B Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The kinesins constitute a large family of microtubule-dependent motor proteins, which are responsible for the distribution of numerous organelles, vesicles and macromolecular complexes throughout the cell (1-3). Individual kinesin members play crucial roles in cell division, intracellular transport, and membrane trafficking events including endocytosis and transcytosis (3,4). Members of the heterotrimeric kinesin II family of microtubule associated motors generally contain two different motor subunits from the KIF3 family, which includes KIF3A, B and C (5). KIF3 isoforms mediate anterograde transport of membrane bound organelles in neurons and melanosomes, transport between the endoplasmic reticulum and the Golgi, and transport of protein complexes within cilia and flagella required for their morphogenesis (6). The human KIF3B gene maps to chromosome 20 and encodes a 747-amino acid protein that is ubiquitously expressed (7). The KIF3B protein is essential for the left-right determination through a mechanism that produces a putative morphogen gradient along the left-right axis in the node (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of KIF3B of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 666-725 mapping near the C-terminus of KIF3B of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 666-725 mapping near the C-terminus of KIF3B of human origin |
| Entrez Gene | 9371 |
|---|
Application
Western blot analysis of KIF3B expression in A2058 (A), T-47D (B) and Caki-1 (C) whole cell lysates and rat brain (D) and mouse brain (E) tissue extracts.| Application Notes | recommended for detection of KIF3B of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
