Application
Western blot analysis of DNA pol λ expression in A549 whole cell lysate.| Product Name | DNA pol λ Antibody (H-172) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 389-560 mapping near the C-terminus of DNA pol λ of human origin |
| Modification Notes | DNA polymerase lambda (pol ¬), also designated DNA polymerase ˚ or Pol ∫2, is a low-fidelity polymerase which plays a role in both spontaneous and DNA damage-induced mutagenesis. Encoded by the POLL gene, pol ¬ is a member of the DNA polymerase type-X family. Pol ¬ extends primer-terminal mispairs opposite nondamaged DNA templates, suggesting that it may assist in extending mismatched base pairs during normal DNA replication. In addition, pol ¬ may play a role in the mutagenic bypass of T-T dimers. Proliferating cell nuclear antigen (PCNA), a protein essential to DNA replication, interacts with pol ¬ and thus influences the ability of pol ¬ to synthesize DNA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DNA pol λ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 389-560 mapping near the C-terminus of DNA pol &lambda of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 389-560 mapping near the C-terminus of DNA pol λ of human origin |
| Entrez Gene | 27343 |
|---|
Application
Western blot analysis of DNA pol λ expression in A549 whole cell lysate.| Application Notes | recommended for detection of DNA pol λ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
