Application
Western blot analysis of yeast recombinant Pbs2 fusion protein.| Product Name | Pbs2 Antibody (y-118) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 551-668 mapping at the C-terminus of Pbs2 of Saccharomyces cerevisiae origin |
| Modification Notes | Yeast cells regulate their internal osmolarity in response to the environment via a MAP kinase cascade (1). Map kinase cascades comprise an essential branch of signal transduction, transmitting extracellular signals to the cytoplasm or nucleus. The core of these cascades consist of a MAP kinase (Mitogen Activated Protein Kinase, also called ERK, for Extracellular-Regulated protein Kinase) as well as one or more up-stream regulatory kinases (MAPKKs or MEKs, for MAP/ERK Kinase) (2,3). High external osmolarity leads to the activation of the MAPKK Pbs2, which activates the MAP kinase Hog1 (4,5). Sho1 also activates Hog1 in response to oxidative stress, and Hog1 (also called Ssk3) is thought to activate a transcription factor that up-regulates the production of osmo-regulatory proteins (6,7). Kex2 is a protease that regulates metabolism by mediating insulin processing (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Pbs2 of Saccharomyces cerevisiae origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 551-668 mapping at the C-terminus of Pbs2 of Saccharomyces cerevisiae origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 551-668 mapping at the C-terminus of Pbs2 of Saccharomyces cerevisiae origin |
Application
Western blot analysis of yeast recombinant Pbs2 fusion protein.| Application Notes | recommended for detection of Pbs2 of Saccharomyces cerevisiae origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
