Application
Western blot analysis of PDX-1 expression in non-transfected: (A) and human PDX-1 transfected: (B) 293T whole cell lysates.| Product Name | PDX-1 Antibody (H-140) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-140 (deletion 20-56) of PDX-1 of human origin |
| Modification Notes | Pancreatic duodenal homeobox-1 protein (PDX-1), also designated insulin promoter factor (IPF1), insulin upstream factor 1 (IUF1), somatostatin trans-activating factor-1 (STF-1) and glucose-sensitive factor (GSF), is a 282 amino acid homeodomain-containing transcription factor present in pancreatic beta-cells. PDX-1 is a key regulator of pancreatic islet development and insulin gene transcription in beta-cells. PDX-1 is expressed in all cells at the early stages of development and is mainly restricted to the pancreas and duodenum in adult. HNF-3b, HNF-1a and SP1 positively regulate the PDX-1 enhancer element. PDX-1 is also regulated by glucagon-like peptide through activation of adenylyl cyclase, which results in an increase in intracellular cAMP activity. The increased levels of cAMP, and the resulting activation of PKA, lead to an increase in PDX-1 transcription and translocation of the protein to the nuclei of beta-cells. PDX-1 binds to the sequence C(C/T) and can heterodimerize with PBX. PDX-1 is phosphorylated by the SAPK2 pathway under high glucose concentrations. Mutations in the PDX-1 gene can cause maturity-onset diabetes of the young and pancreatic agenesis. The gene which encodes PDX-1 maps to human chromosome 13q12.1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of PDX-1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-140 (deletion 20-56) of PDX-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-140 (deletion 20-56) of PDX-1 of human origin |
| Entrez Gene | 3651 |
|---|
Application
Western blot analysis of PDX-1 expression in non-transfected: (A) and human PDX-1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of PDX-1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
