Application
Western blot analysis of CX3CR1 expression in Hela whole cell lysate.Application
Western blot analysis of CX3CR1 expression in THP-1 whole cell lysate.Application
Western blot analysis of CX3CR1 expression in J774A.1 (A), THP-1 (B), RAW 264.7 (C), HUT 78 (D) and LPS treated HL-60 (E) whole cell lysates.| Product Name | CX3CR1 Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 131-200 mapping within an internal region of CX3CR1 of human origin |
| Modification Notes | Chemokines are chemoattractant proteins that are divided into subfamilies based upon cysteine signature motifs termed C, CC, CXC and CX3C. Fractalkine, also designated CX3CL1, contains the CX3C motif and is widely ex-pressed in brain and upregulated in endothelial cells in response to inflammatory signals, such as LPS, IL-1, TNF and CD40L. Fractalkine exists both as a membrane-bound form and as a chemotactic soluble form, and binds its cognate receptor, CX3CR1, with high affinity, to induce leukocyte adhesion and migration or chemotactic functions. CX3CR1, previously designated V28 and chemokine beta receptor-like 1 (CMKBRL1), is expressed in neutrophils, monocytes, T lymphocytes and several organs including brain. CX3CR1 also functions with CD4 as a co-receptor for the HIV-1 virus envelope protein, and patients homozygous for a variant haplotype of CX3CR1 progress to AIDS more rapidly than those with other haplotypes. CX3CR1 may also be involved in the pathogenesis of atherosclerotic coronary artery disease (CAD) and is considered a potential drug target for therapeutic intervention of endothelium-related inflammatory diseases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CX3CR1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 131-200 mapping within an internal region of CX3CR1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 131-200 mapping within an internal region of CX3CR1 of human origin |
| Entrez Gene | 1524 |
|---|
Application
Western blot analysis of CX3CR1 expression in Hela whole cell lysate.
Application
Western blot analysis of CX3CR1 expression in THP-1 whole cell lysate.
Application
Western blot analysis of CX3CR1 expression in J774A.1 (A), THP-1 (B), RAW 264.7 (C), HUT 78 (D) and LPS treated HL-60 (E) whole cell lysates.| Application Notes | recommended for detection of CX3CR1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
