Application
Western blot analysis of dHAND expression in human ovary (A), human placenta (B) and human heart (C) tissue extracts.Application
Western blot analysis of dHAND expression in ES-2 whole cell lysate.Application
Western blot analysis of dHAND expression in ES-2 (A) and HeLa (B) whole cell lysates.| Product Name | dHAND Antibody (H-110) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-110 of dHAND of human origin |
| Modification Notes | dHAND (for deciduum, heart, autonomic nervous system and neural crest derivatives; also designated HAND2) and eHAND (also designated HAND1, HXT or Thing1) are members of a subclass of basic-helix-loop-helix transcription factors that are involved in cardiac development. dHAND and eHAND are expressed in the heart after cardiac looping and participate in left-right cardiac asymmetry. dHAND is expressed predominantly on the right side of the looped heart tube and in the pulmonary ventricle, where it activates transcription of various genes, including Ufd1 (for ubiquitin fusion degradation) and Cdc45. In addition, dHAND is expressed in sympathetic neurons and chromafin cells throughout embryonic and fetal development, and mediates neural crest development. eHAND expression is primarily observed on the left side and in the systemic ventricle, suggesting that these proteins are involved in the development of segments of the heart tube, which give rise to specific heart chambers during cardiogenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of dHAND of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-110 of dHAND of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-110 of dHAND of human origin |
| Entrez Gene | 9464 |
|---|
Application
Western blot analysis of dHAND expression in human ovary (A), human placenta (B) and human heart (C) tissue extracts.
Application
Western blot analysis of dHAND expression in ES-2 whole cell lysate.
Application
Western blot analysis of dHAND expression in ES-2 (A) and HeLa (B) whole cell lysates.| Application Notes | recommended for detection of dHAND of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
