Application
Western blot analysis of GRB2 expression in PC-12 whole cell lysate.| Product Name | GRB2 Antibody (H-68) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-68 mapping at the N-terminus of GRB2 of human origin |
| Modification Notes | The superfamily of GTP binding proteins, of which Ras proteins are prototypes, has been implicated in a broad range of biological activities. A family of guanine nucleotide releasing factors (GRFs) activate Ras in mammalian cells and growth factor receptor-bound protein 2 (GRB2), an adaptor protein (also referred to as Sem 5) that appears to mediate the interaction of GRFs with activated receptor molecules. GRB2 forms a complex with activated EGFR (epidermal growth factor receptor) and the Ras-specific guanine nucleotide exchange factor SOS1, and, together, they regulate the growth factor-induced activation of Ras. GRB2 exhibits both structural and functional homology to the C. elegans protein sem-5. GRB2 is necessary during embryogenesis for the differentiation of endodermal cells and formation of the epiblast. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GRB2, and, to a lesser extent, Grap of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-68 mapping at the N-terminus of GRB2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-68 mapping at the N-terminus of GRB2 of human origin |
| Entrez Gene | 2885 |
|---|
Application
Western blot analysis of GRB2 expression in PC-12 whole cell lysate.| Application Notes | recommended for detection of GRB2, and, to a lesser extent, Grap of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
