Application
Western blot analysis of Nectin 1 expression in IMR-32 whole cell lysate (A) and human brain (B), mouse brain (C) and rat brain (D) tissue extracts.| Product Name | Nectin 1 Antibody (H-62) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 451-512 mapping near the C-terminus of Nectin 1 of human origin |
| Modification Notes | Nectin is a Ca2+-independent homophilic cell adhesion molecule that belongs to the immunoglobulin superfamily. Human Nectin is identical to the poliovirus receptor-related protein (PRR) and is identified to be the alphaherpesvirus entry mediator. Nectin constitutes a family consisting of at least nectin 1, 2 and 3. Nectin 2 and 3 are ubiquitously expressed, whereas nectin 1 is abundantly expressed in the brain. Nectin 1 exists as nectin 1å and 1∫/HIgR, produced by alternative splicing. The cytoplasmic regions of Nectin 1å, but not Nectin 1∫/HIgR, have a C-terminal conserved motif (E/A-X-Y-V). This motif interacts with the PDZ domain of the F-Actin-binding protein, afadin, through which it is linked to the Actin cytoskeleton. Nectin 1, also designated HveC/ PRR1, allows the entry of herpes simplex virus type 1 (HSV-1) and HSV-2 into mammalian cells. The interaction of virus envelope glycoprotein D (gD) with nectin 1 is an essential step in the process leading to membrane fusion; the gD binding site is located at the first Ig-like domain of Nectin 1. Both the transinteraction of nectin and the interaction of nectin with afadin are necessary for their co-localization with E-cadherin and catenins at adherens junctions. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Nectin 1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 451-512 mapping near the C-terminus of Nectin 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 451-512 mapping near the C-terminus of Nectin 1 of human origin |
| Entrez Gene | 5818 |
|---|
Application
Western blot analysis of Nectin 1 expression in IMR-32 whole cell lysate (A) and human brain (B), mouse brain (C) and rat brain (D) tissue extracts.| Application Notes | recommended for detection of Nectin 1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
