Application
Western blot analysis of Swe1 expression in S. cerevisiae whole cell lysate.| Product Name | Swe1 Antibody (y-311) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-311 representing full length Swe1 of Saccharomyces cerevisiae origin |
| Modification Notes | Cell cycle progression is controlled at a point late in G1 designated Start. Passage through Start requires the activity of the cyclin-dependent protein kinase Cdc28 (1,2). Transition from G1 to S phase requires the association of Cdc28 with members of the G1 cyclin family (3). The G2 to M phase transition requires the M phase cyclins as well as the G2 cyclins (4). The S phase cyclins coordinate DNA replication with cytokinesis (5). Expression of the cyclins is controlled via ubiquitin-mediated proteolysis (6). Cdc28 is regulated by the protein kinase Swe1 (7). This protein, a homolog of the Saccharomyces pombe Wee1 protein, phosphorylates Cdc28 and inhibits its activity (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| recommended for detection of Swe1 of Saccharomyces cerevisiae origin by WB, IP and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-311 representing full length Swe1 of Saccharomyces cerevisiae origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-311 representing full length Swe1 of Saccharomyces cerevisiae origin |
Application
Western blot analysis of Swe1 expression in S. cerevisiae whole cell lysate.| Application Notes | recommended for detection of Swe1 of Saccharomyces cerevisiae origin by WB, IP and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
