Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing strong nuclear staining and moderate cytoplasmic staining of glomerular cells and cells in tubules.Application
Western blot analysis of FKBP51 expression in non-transfected 293T: (A), human FKBP51 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of FKBP51 expression in non-transfected: (A) and mouse FKBP51 transfected: (B) 293T whole cell lysates and mouse thymus tissue extract (C).Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing nuclear staining of glandular and lymphoid cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of FKBP51 expression in HL-60 (A) and HeLa (B) whole cell lysates.Application
Western blot analysis of FKBP51 expression in mouse thymus (A) and rat thymus (B) tissue extracts and HeLa (C), Ca Ski (D) and Jurkat (E) whole cell lysates.| Product Name | FKBP51 Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 358-457 mapping at the C-terminus of FKBP51 of human origin |
| Modification Notes | The immunophilins are a highly conserved family of cis-trans peptidyl-prolyl isomerases that bind to and mediate the effects of immunosuppressive drugs, such as cyclosporin, FK506 and rapamycin (1). Several related immunophilins, FKBP12, FKBP51 and FKBP52, are characterized as cytosolic FK506-binding proteins, and following ligand binding, they functionally inhibit the phosphatase activity of calcineurin (2,3). The ubiquitously expressed FKBP12 also associates with the cytoplasmic domain of the TGFß-type I receptor, where it stabilizes the inactive conformation of the receptor and blocks the activation of the TGFß pathway (4). FKBP51 and FKBP52 are two highly related proteins (5,6). FKBP51 is predominantly expressed in T cells and is induced by glucocorticoids (5). FKBP51 mediates the effects of FK506 and rapamycin by inhibiting intracellular calcineurin activity, and by blocking T-cell activation and proliferation (7). FKBP52, known also as FKBP-59 or heat shock protein 56, is expressed in a variety of tissues and can also associate with the heat shock protein (hsp90) in mature steroid receptor complexes (6,8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of FKBP51 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 358-457 mapping at the C-terminus of FKBP51 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 358-457 mapping at the C-terminus of FKBP51 of human origin |
| Entrez Gene | 2289 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing strong nuclear staining and moderate cytoplasmic staining of glomerular cells and cells in tubules.
Application
Western blot analysis of FKBP51 expression in non-transfected 293T: (A), human FKBP51 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of FKBP51 expression in non-transfected: (A) and mouse FKBP51 transfected: (B) 293T whole cell lysates and mouse thymus tissue extract (C).
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing nuclear staining of glandular and lymphoid cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of FKBP51 expression in HL-60 (A) and HeLa (B) whole cell lysates.
Application
Western blot analysis of FKBP51 expression in mouse thymus (A) and rat thymus (B) tissue extracts and HeLa (C), Ca Ski (D) and Jurkat (E) whole cell lysates.| Application Notes | recommended for detection of FKBP51 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
