Application
Western blot analysis of MICA expression in non-transfected: (A) and human MICA transfected: (B) 293T whole cell lysates.Application
Western blot analysis of MICA/B expression in HeLa whole cell lysate.Application
Western blot analysis of MICA and MICB expression in MCF7 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.| Product Name | MICA/B Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | MICA and MICB are stress-induced antigens that are related to major histocompatibility complex (MHC) class I molecules. MICA and MICB are frequently expressed in epithelial tumors. These highly glycosylated cell surface proteins are stably expressed without conventional class I peptide ligands or association with ∫-2-Microglobulin. The expression is induced on proliferating or heat shock-stressed epithelial cells. MICA and MICB are broadly recognized by intestinal epithelial V∂1 ©∂ T cells expressing variable TCRs, suggesting that these antigens may play a central role in the signaling of cellular distress to evoke immune responses in the intestinal epithelium. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MICA and MICB of human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of MICA of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of MICA of human origin |
| Entrez Gene | 4276 |
|---|
Application
Western blot analysis of MICA expression in non-transfected: (A) and human MICA transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of MICA/B expression in HeLa whole cell lysate.
Application
Western blot analysis of MICA and MICB expression in MCF7 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.| Application Notes | recommended for detection of MICA and MICB of human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
