Application
Western blot analysis of IRX3 expression in non-transfected: (A) and human IRX3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of IRX3 expression in non-transfected: (A) and human IRX3 transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).| Product Name | IRX3 Antibody (M-147) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The Iroquois homeobox gene family of transcription factors regulate aspects of embryonic development including anterior/posterior and dorsal/ventral axis patterning in the central nervous system. The Iroquois family are clustered on two loci, IRXA and IRXB, which map to chromosomes 8 and 13 in mice. The IRXA group includes IRX, IRX2 and IRX4; the IRXB group comprises IRX3, IRX5 and IRX6. IRX1 and IRX2 are both widely expressed during development in the lung epithelium and also in the ventricular septum. IRX1 and IRX2 also play a role in digit formation (E11.5–E14.5). The IRX gene family members are each expressed in a distinct pattern during mouse heart development. Specifically, IRX1 and IRX2 are expressed in the ventricular septum and IRX3 is expressed in the ventricular trabeculated myocardium. In addition, IRX4 is expressed in the linear heart tube and the AV canal; IRX5 is expressed in the endocardium lining the ventricular and atrial myocardium. Furthermore, the IRX4 gene may modulate cardiac development and function. Although the heart of IRX4(-) mice appears to develop normally, adult IRX4(-) mice exhibit cardiomyopathy, including cardiac hypertrophy and decreased contractility. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of IRX3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 361-507 mapping at the C-terminus of IRX3 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 361-507 mapping at the C-terminus of IRX3 of mouse origin |
| Entrez Gene | 79191 |
|---|
Application
Western blot analysis of IRX3 expression in non-transfected: (A) and human IRX3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of IRX3 expression in non-transfected: (A) and human IRX3 transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).| Application Notes | recommended for detection of IRX3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
