Application
Immunofluorescence staining of methanol-fixed C32 cells showing cytoplasmic localization.Application
Western blot analysis of CD63 expression in T24 (A), SK-MEL-28 (B), CCD-1064Sk (C), C32 (D) and THP-1 (E) whole cell lysates.| Product Name | CD63 Antibody (H-193) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 (also known as LAMP-3, Melanoma-associated antigen ME491, TSPAN30, MLA1 and OMA81H) is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CD63 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 45-238 mapping at the C-terminus of CD63 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 45-238 mapping at the C-terminus of CD63 of human origin |
| Entrez Gene | 967 |
|---|
Application
Immunofluorescence staining of methanol-fixed C32 cells showing cytoplasmic localization.
Application
Western blot analysis of CD63 expression in T24 (A), SK-MEL-28 (B), CCD-1064Sk (C), C32 (D) and THP-1 (E) whole cell lysates.| Application Notes | recommended for detection of CD63 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
