Application
Western blot analysis of connexin 43 expression in non-transfected: (A) and human connexin 43 transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).Application
Antibodies tested include connexin 43 (H-150): (A,B), p-connexin 43 (Ser279/282)-R: (C,D) and p-connexin 43 (Ser 255): (E,F).Application
Immunofluorescence staining of methanol-fixed SK-N-SH cells showing membrane localization.Application
Western blot analysis of connexin 43 expression in mouse (A) and rat (B) brain extracts.| Product Name | connexin 43 Antibody (H-150) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The connexins are a group of gap junction proteins which form a hexamer to compose a connexon. Clusters of connexons form a gap junction through which low molecular weight proteins may diffuse from cell to cell. Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and gap junction communication. In Src transformed cells, there is a decrease in gap junctional communication, which appears to be associated with tyrosine phosphorylation of connexin 43. Activated c-Src phosphorylates the C-terminal tail of connexin 43 on Tyr 265, resulting in a stable interaction between both proteins, which leads to inhibition of gap junctional communication. In addition to tyrosine phosphorylation, connexin 43 has also been shown to be phosphorylated on serine in the absence of Src kinases and on both serine and tyrosine in cells expressing Src kinases, such as c-Src and/or pp60v-Src. In human vascular endothelial cells, connexin 43 is posttranslationally modified during mitosis. Mitosis-specific phosphorylation of connexin 43 correlates with the transient loss of gap junction intercellular communication and redistribution of connexin 43. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of connexin 43 of mouse, rat, human, zebrafish and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 233-382 mapping at the C-terminus of connexin 43 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 233-382 mapping at the C-terminus of connexin 43 of human origin |
| Entrez Gene | 2697 |
|---|
Application
Western blot analysis of connexin 43 expression in non-transfected: (A) and human connexin 43 transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).
Application
Antibodies tested include connexin 43 (H-150): (A,B), p-connexin 43 (Ser279/282)-R: (C,D) and p-connexin 43 (Ser 255): (E,F).
Application
Immunofluorescence staining of methanol-fixed SK-N-SH cells showing membrane localization.
Application
Western blot analysis of connexin 43 expression in mouse (A) and rat (B) brain extracts.| Application Notes | recommended for detection of connexin 43 of mouse, rat, human, zebrafish and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
