Application
Western blot analysis of Espin expression in non-transfected: (A) and mouse Espin transfected: (B) 293T whole cell lysates.| Product Name | Espin Antibody (C-12) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Espin (ESPN), also known as autosomal recessive deafness type 36 protein (DFNB36), is an 854 amino acid cytoplasmic protein that contains nine ANK repeats and one WH2 domain. The WH2 domain of Espin binds actin monomers and mediates the assembly of the actin bundle. This interaction plays a major role in the moderation of the organization, dynamics and signaling capacities of the actin filament-rich specializations that regulate sensory transduction in various sensory cells. Defects in Espin are the cause of non-syndromic sensorineural deafness autosomal recessive type 36 (DFNB36), a sensorineural hearing loss caused by damage to the neural receptors of the inner ear, the nerve pathways to the brain or the region of the brain responsible for sound. In humans, Espin is expressed as two isoforms produced by alternative splicing and has been found to interact with IRSp53 and Profilin-2. In rodents, four major isoforms ranging from approximately 110 to 25 kDa have been identified with additional splice variants possible. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Espin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Espin of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Espin of human origin |
| Entrez Gene | 83715 |
|---|
Application
Western blot analysis of Espin expression in non-transfected: (A) and mouse Espin transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Espin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
